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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Apr 26;1844(9):1453–1462. doi: 10.1016/j.bbapap.2014.04.015

Figure 8. Titrations of hCBS with SAM in the absence or presence of 200 mM NaCl by ITC.

Figure 8

A) and B) show raw calorimetric data, while C and D) display binding isotherms, with the corresponding fittings to two (solid line) or one (dashed line) independent type of sites. Experiments were performed using 8.5 μM (no salt) and 20 μM enzyme (200 mM NaCl) calculated per hCBS tetramer and 0.7 mM (no salt) or 1.0 mM SAM (200 mM NaCl) .