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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Apr 26;1844(9):1453–1462. doi: 10.1016/j.bbapap.2014.04.015

Figure 9. Effect of SAM on hCBS thermal denaturation in the absence or presence of 200 mM NaCl.

Figure 9

Thermal denaturation profiles in the absence (A) and presence (B) of salt. The concentrations of SAM are indicated alongside the DSC profiles. C) SAM concentration dependence of the Tm of the RD in the absence (closed circles) or presence of 200 mM NaCl (open circles). Fitting errors are smaller than the symbols. Lines are meant to guide the eye and have no theoretical meaning. D) Plots used to calculate ν (the number of SAM molecules released per CBS tetramer prior to the rate-limiting step of denaturation) values of 1.9±0.1 (no salt; closed circles) and 2.4±0.2 (200 mM NaCl; open circles) using the procedure previously described [4, 45].