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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jan 27;0:49–63. doi: 10.1016/j.jchromb.2014.01.026

Fig. 11.

Fig. 11

Analysis of split-peak data according to Eq. (12) for injections of rabbit immunoglobulin G (IgG) onto protein A columns prepared by various immobilization methods and supports. The conditions for each plot were as follows: Schiff base method, 500 Å pore size silica, 22 μg IgG (●); Schiff base method, 500 Å pore size support, 11 μg IgG (●); Schiff base method, 50 Å pore size support, 15 μg IgG (▲); carbonyldiimidazole method, 500 Å pore size support, 2.7 μg IgG (■); and ester/amide method, 500 Å pore size support, 8.2 μg IgG (◆). Reproduced with permission from Ref. [97].