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. Author manuscript; available in PMC: 2015 Jul 22.
Published in final edited form as: Oncogene. 2014 Jan 27;34(4):496–505. doi: 10.1038/onc.2013.567

Figure 3. KSHV vFLIP-induced G1 arrest is driven by RelA.

Figure 3

(A) HeLa-G cell line (Ctrl) and its progeny cell lines (sh-RelA, sh-RelB sh-p100, and sh-c-Rel) wherein RelA, RelB plus p100, and c-Rel respectively had been stably knocked down by small hairpin RNAs (shRNAs). In sh-RelB-p100 cell line, both RelB and p100 were knocked down to block the alternative NF-κB pathway. These cells were transduced with LV-neo or LV-vFLIP-Flag-neo, and selected with G418 (800µg/ml). The transduced cells were harvested 5 days after selection and analyzed by immunoblotting using antibodies indicated on the right. (B) Phase contrast photographs of LV-neo or LV-vFLIP-Flag-neo-transduced cells knocked down for various NF-κB family members on day 5 after G418 selection.