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. Author manuscript; available in PMC: 2014 Jul 28.
Published in final edited form as: Acta Biomater. 2012 Dec 20;9(5):6468–6480. doi: 10.1016/j.actbio.2012.12.015

FIGURE 3. Effect of film stiffness and RGD fonctionalization on cytoskeletal organization, focal adhesions and migration.

FIGURE 3

(A) Staining of actin cytoskeleton (red) after 4 h of culture. (B) Staining of phosphorylated focal adhesion kinase (pFAK Y397, green) after 4 h of culture. (C) Myoblast migration measured over 5 h after seeding. (D) Effect of blocking β1 and/or β3 integrins using siRNA: quantification of the cell area after 4 h of adhesion (* p < 0.05 compared to scrambled siRNA). Focal adhesions/complexes are indicated by white arrowheads.