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. 2014 Jul 14;87(1040):20140184. doi: 10.1259/bjr.20140184

Figure 3.

Figure 3.

Platelet factor 4 (PF4)–repressed human bone marrow mesenchymal stem cell (hBMSC) apoptosis and induced S phase arrest in vitro. (a–f) hBMSCs were pre-incubated with 1 μg ml−1 of PF4 for 12 h or 10 μM WR-2721 for 30 min before 500- and 0-cGy ionizing irradiation. Cell apoptosis was analysed by FACSCalibur™ (BD Immunocytometry Systems, San Jose, CA) on the second day (a, b); on the fourth day (c, d); and on the sixth day (e, f). (g) Short-term S phase arrest regulation by PF4. Cell cycle was analysed by FACSCalibur at 20 h. (h) Qualitative measurement of (g). Bars, means ± standard deviation. *p < 0.05; **p < 0.01; #p < 0.001; n = 5. IR, ionizing irradiation; PI, propidium iodide.