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. Author manuscript; available in PMC: 2015 Jan 24.
Published in final edited form as: Nat Commun. 2014 Jul 24;5:4467. doi: 10.1038/ncomms5467

Figure 5. Loss of PfHAD1 is required for FSM resistance.

Figure 5

Data shown are representative of at least three independent experiments. (a) Immunoblot of the parent strain, FSMR strain AM1, and AM1 Hsp110:PfHAD1-GFP. Full blots are shown in Supplementary Fig. 3. Marker units are kilodaltons (kDa). Top panel was probed with anti-PfHAD1 antisera, and the bottom panel was probed with anti-heat shock protein 70 (hsp70) antisera as a loading control. Expected approximate protein masses: native PfHAD1, 33 kDa; PfHAD1-GFP, 60 kDa; hsp70, 74 kDa. (b) FSM IC50s of the parent strain, FSMR strain AM1, and AM1 Hsp110:PfHAD1-GFP. Displayed are the means ± standard error of the mean (SEM). The parent strain has a FSM IC50 of 1.1 ± 0.2 μM, while AM1 has an IC50 of 5.5 ± 1.5 μM. Expression of PfHAD1-GFP in AM1 results in an IC50 of 1.4 ± 0.2 μM. Data shown are mean is normalized such that 100% growth is defined by that of untreated cells, and 0% growth is defined as the smallest value in each data set.