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. Author manuscript; available in PMC: 2014 Jul 28.
Published in final edited form as: Curr Med Chem. 2013;20(33):4131–4141. doi: 10.2174/09298673113209990248

Fig. (6).

Fig. (6)

1,25(OH)2D3 inhibits VDR gene silenced tumor cell growth in wild-type mice, but not in p27kip1 gene knock-out mice. C57BL background derived Hepa1–6 cells were pre-treated with VDR shRNA and implanted to the p27+/+ or p27−/− mice, addition with 1,25(OH)2D3 or PBS intraperitoneal injection every day. (A) Gross appearances of representative livers with transplanted tumors in either normal or 1,25(OH)2D3-treated p27+/+ and p27−/− mice (n=6). (B) Serum IL-6 and TNF-α level. (C) Immunoblotting results of cell cycle, apoptosis-related, and STAT3 phosphorylation proteins in transplanted tumors from either 1,25(OH)2D3-untreated or -treated p27+/+ and p27−/− mice. (D) PCNA immunohistochemistry of representative tumor slices (magnification 200x). (E) TUNEL staining of representative tumor slices (magnification 400x). (F) p-STAT3 immunohistochemistry of representative tumor slices from either vehicle or 1,25(OH)2D3-treated p27+/+ and p27−/− mice (magnification 400x). The results are representative of at least 3 independent samples. *p<0.05, **p<0.005, ***p<0.001.