Skip to main content
. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Behav Neurosci. 2013 Dec;127(6):923–931. doi: 10.1037/a0034839

Figure 1.

Figure 1

A. Placement of infusion cannulae in the olfactory bulb. Guide cannulae are placed at about 0.5 mm above the center of the olfactory bulb in such a manner that injection cannulae, protruding 0.5 mm beyond the guide cannulae target the approximate center of the olfactory bulb. B. Sagittal view of CD-1 mouse olfactory bulbs after bilateral infusion of 2μL methylene blue dye (1mg/mL). Perfusion throughout the bulbs is evident with very little spread beyond the bulbs into the rest of the brain. C. Schematic depiction of the habituation and memory behavioral trial experimental setup. MO is the mineral oil blank presented prior to the habituation presentations (H1-H4). In the cannulation studies, the behavioral trials were preceded by a 10 minute infusion and a 30 minute wait period. Mice are first presented with mineral oil only (MO), followed by four presentations of the habituation odor (H1-H4) at 5 minute ITIs. The habituation odor is presented again 30 or 60 minutes after the last habituation trial.