Table 2. Hit Rates in the Primary High-Throughput Screen (HTS).

% of GCPII Bindinga	mP	Number of hits at 1 μMb	Number of hits at 60 μMc	Autofluorescent Compoundsd
>90%	<88	0	6	3
>75%	<130	0	12	5
>50%	<215	10	65	19
>30%	<281	15	119	28
>25%	<300	18	166	30

A library of nearly 20 000 compounds was screened and data were normalized with the b-score algorithm. GCPII, glutamate carboxypeptidase II.

^aHits were identified using different cutoffs based on the percentage of probe displacement from the GCPII protein according to mP values.

^bNumber of hits in the 1-μM screen.

^cNumber of hits in the 60-μM screen.

^dNumber of active compounds in the HTS showing at least 5 times higher autofluorescence than the background.