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. Author manuscript; available in PMC: 2014 Jul 28.
Published in final edited form as: Anal Biochem. 2013 Aug 9;443(1):1–12. doi: 10.1016/j.ab.2013.08.002

Fig.4.

Fig.4

Cloning results and binding rates of the released miRNAs from the pull-down of mouse liver cell miRNA pool and 3′ end biotin-labeled RNA of Smad4 3′UTR. (A) Species and quantity of the released miRNAs from the first pull-down. The results are from 22 random colonies. (B) Species and quantity of the released miRNAs from the second pulldown. The results are from 38 random colonies. (C) Binding rates of 8 released miRNAs. miR-150 had the highest binding rate at approximately 40%. The binding rate data are from biological duplicates and technical duplicates in each. Error bars represent standard deviations.