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. Author manuscript; available in PMC: 2014 Jul 28.
Published in final edited form as: Anal Biochem. 2013 Aug 9;443(1):1–12. doi: 10.1016/j.ab.2013.08.002

Fig.5.

Fig.5

Luciferase reporter assay showing miRNA mimic/inhibitor effects on gene 3′UTR. (A) Luciferase reporter assay showing the miRNA mimic/inhibitor effects on TCF8 3′UTR in A549 cells. The miRNA mimics/inhibitors were chosen from TCF8 3′UTR pull-down. (B) Luciferase reporter assay showing the miRNA mimic/inhibitor effects on Smad4 3′UTR in CRL-1995 cells. The miRNA mimics/inhibitors were chosen from Smad4 3′UTR pull-down. “Pre-negative control” represents miRNA precursor negative control, and “inhibitor-negative control” represents miRNA antisense inhibitor negative control.