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. Author manuscript; available in PMC: 2015 May 22.
Published in final edited form as: Cell Rep. 2014 May 1;7(4):1143–1155. doi: 10.1016/j.celrep.2014.03.061

Figure 3. Activated Rab7 rescues cancer phenotype in Plac8 KD cells.

Figure 3

Murine mp53/Ras cells (A, C, E) and human CAPAN-2 cells (B, D, F) were grafted into CD-1 nude mice and tumor growth was monitored. Mean values of tumor volume +/− SD and student’s T-test at final time-point are indicated (*** p<0.001; ** p<0.01; *p<0.05; n≥9 tumors per condition). Cells expressing 3xFlag-tagged Rab7DN (Rab7T22N) or vector control (Vec) (A&B), 3xFlag-tagged Rab5a DN (Rab5aS34N) or Vec (C&D), and 3xFlag-tagged Rab7 DA,, Plac8 shRNA, Plac8 shRNA with 3xFlag-tagged Rab7 DA or Vec (E&F) were used. Corresponding cell lysates were immunoblotted for p62, LC3, Rab5 or Rab7 (A–F), and Plac8 in E&F (β-tubulin loading control).