Table 2.

Pathway enrichment analysis of genes whose expression was significantly affected by drought stress and by the nNOS transgenic effect in Arabidopsis

Groups	Pathways	nNOS drought versus nNOS control		WT drought versus WT control		nNOS drought versus WT drought		nNOS control versus WT control
		NF	P-value	NF	P-value	NF	P-value	NF	P-value
I	PS	5.49	0.0000	2.11	0.1740	8.15	0.1090	6.68	0.0000
	Metal handling	4.54	0.0041	15.77	0.0000	–	–	–	–
	Hormone metabolism	4.18	0.0000	2.41	0.0260	–	–	1.52	0.0440
	Redox	2.15	0.0390	11.4	0.0000	–	–	1.96	0.0520
	Stress	2.09	0.0000	3.37	0.0000	2.73	0.1300	1.45	0.0140
	Miscellaneous	1.66	0.0011	3.7	0.0000	4.22	0.0110	1.34	0.0200
	Mitochondrial electron transport	1.50	0.1820	1.44	0.3480	–	–	2.28	0.0470
	Oxidative Pentose phosphate	2.43	0.2750	7.03	0.1240	–	–	2.22	0.2900
	Lipid metabolism	1.05	0.1610	1.01	0.2730	–	–	1.12	0.1440
II	Secondary metabolism	1.53	0.0650	0.98	0.2730	–	–	2.48	0.0006
	RNA	1.48	0.0005	0.64	0.0450	1.65	0.1690	1.01	0.0620
	Transport	1.40	0.0330	1.7	0.0520	–	–	0.53	0.0200
	Development	0.95	0.1260	1.38	0.1400	4.26	0.0680	1.39	0.0440
	Cofactor and vitamin metabolism	0.93	0.3700	–	–	–	–	3.4	0.0240
	Major CHO metabolism	0.74	0.3530	–	–	–	–	2.72	0.0440
	Protein	0.7	0.0016	0.54	0.0049	0.69	0.2400	1.08	0.0380
	Signalling	0.66	0.0350	0.64	0.1220	1.23	0.3700	1.11	0.0770
	Amino acid metabolism	0.58	0.1890	0.83	0.3640	–	–	1.58	0.0930
	Cell wall	0.41	0.0440	1.19	0.2170	–	–	1.88	0.0081
	Cell	0.36	0.0095	0.52	0.1600	6.07	0.0110	0.66	0.0630
III	Not assigned	0.93	0.0240	0.53	0.0000	0.56	0.0730	0.89	0.0096
	DNA	0.07	0.0000	0.13	0.0000	–	–	0.11	0.0000

Differentially expressed genes (i.e. with P value ≤0.05 and log₂ fold-change ≥1 or log₂ fold-change ≤ –1) were annotated using the Classification SuperViewer Tool and MapMan. shading scales of NF are as follows:

≥3 2–3 1–2 0.5–1 ≤0.5