Fig. 6.

MALDI-TOF mass spectrometry analysis of the relative abundance of xyloglucan oligosaccharides released by xyloglucan-specific endoglucanase. (XEG). (A) Relative proportions of xyloglucan subunits generated from cell wall preparations of wild-type (WT, white bar) and 35S::OsXXT1 Arabidopsis (black bar) leaves digested with XEG. Results are expressed as the percentile of the areas of the corresponding peaks for each subunit on high-performance anion exchange chromatography (HPAEC). Arabidopsis were grown in growth chamber for two months and leaves of each of the plants were sampled as a biological replicate. All analyses were performed on three plant preparations. Significant differences are indicated with an asterisk. nd, not detected. (B) MALDI-TOF mass spectrometry of XEG-generated xyloglucan fragments from the hemicellulosic fractions of wild-type (WT, Columbia-0), Arabidopsis xxt1 xxt2 double mutant, and complemented Arabidopsis xxt1 xxt2 double mutant with 35S::OsXXT1 (35S::OsXXT1). Xyloglucan subunit is described from the nomenclature introduced by Fry et al. (1993). WT (top), Arabidopsis xxt1 xxt2 (middle) and 35S::OsXXT1 (bottom). Xylopentaose was used as a standard control (std).