Table 1.
Enzyme kinetics of recombinant PfaGT1-316 as determined by Lineweaver-Burke plotData represent mean and SD from three independent batches of protein purification, each with at least three technical replicates. ND, K m for kaempferol could not be determined due to poor activity.
| Substrate | V max (pkat) | K m (mM) | k cat (s–1) | k cat/K m (mM–1 s–1) |
|---|---|---|---|---|
| Caffeic acid | 15.85±4.50 | 0.22±0.09 | 6.49±1.84 | 31.3±4.3 |
| 4-Coumaric acid | 15.07±2.55 | 0.62±0.12 | 6.17±1.05 | 10.1±0.3 |
| 4-Hydroxybenzoic acid | 10.61±2.95 | 0.72±0.33 | 4.35±1.21 | 6.6±2.0 |
| 2-Coumaric acid | 8.99±3.44 | 0.11±0.05 | 3.68±1.41 | 35.9±3.9 |
| Ferulic acid | 7.93±1.27 | 0.15±0.02 | 3.25±0.52 | 22.1±1.6 |
| Sinapic acid | 7.55±2.12 | 0.13±0.05 | 3.09±0.87 | 24.7±3.0 |
| Benzoic acid | 3.68±0.89 | 1.59±0.41 | 1.51±0.36 | 1.0±0.1 |
| Cinnamic acid | 3.42±0.59 | 0.30±0.07 | 1.40±0.24 | 4.7±0.9 |
| Naringenin | 1.99±0.31 | 0.22±0.10 | 0.81±0.13 | 4.3±1.6 |
| Kaempferol | 0.55±0.13 | ND | 0.23±0.05 | ND |