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. 2014 May 12;65(15):4241–4253. doi: 10.1093/jxb/eru195

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — AtPCS2 expression results in constitutive PC accumulation and rescues growth of cad1-3 on Cd-contaminated soil. Ten-day-old A. thaliana seedlings of wild-type Col-0, the AtPCS1 mutant cad1-3, and lines overexpressing AtPCS1 or AtPCS2 in the cad1-3 background were transferred to control soil or to artificially Cd-contaminated soil. (A) Pictures of plants after 24 d of growth on control soil (top) or soil spiked with 7.5mg Cd²⁺ kg^–1 (bottom). (B) Leaf area was quantified weekly during the course of the experiment. (C, D) Leaf material was pooled and assayed for PC accumulation via UPLC-ESI-QTOF-MS (C) and Cd accumulation using inductively coupled plasma optical emission spectroscopy (D). Data represent means±SD of two independent experiments (n=4). Statistical analyses were performed via two-way ANOVA and data were grouped with Tukey’s 95% confidence interval. Percentage values were transformed prior to analysis.