Fig. 7.

PC accumulation in Schizosaccharomyces pombe Δpcs cells expressing AtPCS1 or AtPCS2. Schizosaccharomyces pombe cells carrying a construct with AtPCS1, AtPCS2, or the empty vector (EV) were grown overnight in EMM containing 20 µM thiamine, i.e. under conditions suppressing PCS expression. The cells were washed and inoculated to an OD₆₀₀ of 0.4 in EMM without thiamine to induce PCS expression in the presence or absence of 10 µM CdCl₂. After 6h of cultivation, the cells were harvested and PCs were extracted, labelled with mBrB, and quantified using UPLC-ESI-QTOF-MS. Data represent means±SD of two independent experiments (n=8).