Table 3.
Genes significantly misregulated in three independent microarray experimentsa in TopoVI mutants
| AGI | Annotation, gene ontology process/function | top6a/caa39 b | hlq/top6b log2FC (this work) | Concordance? |
|---|---|---|---|---|
| At5g42650 | CYP74A/DELAYED DEHISCENCE2; allene oxide synthase | Singlet induction TOP6A dependent | –0.67 | Yes |
| At5g47370 | HAT2, homeodomain transcription factor induced by auxin; root morphogenesis related | Singlet repressed, TOP6A independent | –0.29 | Yes |
| At5g57560c | TOUCH4/cell wall modifying; rapidly induced by environmental stimuli | TOP6A repressed, independent of singlet induction | 1.29 | Not for bin3 |
| At1g01120 | KCS1, 3-ketoacyl-CoA synthase, critical for fatty acid elongation in wax biosynthesis | Singlet induced, TOP6A independent | –0.49 | Not for singlet |
| At2g44940 | DREB subfamily A-4 of Ethylene Response Factor/APETELA2 domain transcription factor | Singlet induced, TOP6A independent | –0.49 | Not for singlet |
| At4g20780 | CALMODULIN LIKE 42, EF hand domain calcium and protein binding; involved in trichome branching, abiotic stress responses | Singlet induced, TOP6A independent | –0.27 | Not for singlet |
| At2g17880 | Chaperone DnaJ heat shock protein, response to sugar | Singlet repressed, TOP6A independent | 0.25 | No |
a Originally reported (Yin et al., 2002) as down-regulated >2-fold in both bin3/top6b and bin5/top6a mutants.
b Genes classified (Simkova et al., 2012) as ‘topo6a/caa39 induced’ (cluster 1); ‘topo6a repressed’ (cluster 2); ‘singlet repressed, TOP6A independent’ (cluster 3); ‘singlet induced TOP6A dependent’ (cluster 4); ‘singlet induced TOP6A repressed’ (cluster 5); ‘TOP6A repressed, independent of singlet induction’ (clusters 6, 8); ‘singlet induced, TOP6A independent’ (cluster 7).
c Reported (Yin et al., 2002) as down-regulated; brassinosteroid induction dependent on BIN3/TOP6B and BIN5/TOP6A.