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. 2014 May 26;65(15):4451–4464. doi: 10.1093/jxb/eru219

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Interactions between PYR/PYL ABA receptors and clade A PP2Cs. Interaction was determined by growth assay on media lacking histidine and adenine (–H, –A), which were supplemented or not with 50 μM ABA (+ABA). Dilutions (10^–1, 10^–2, and 10^–3) of saturated cultures were spotted onto the plates. (A) Interaction of tomato receptors with Arabidopsis PP2Cs. (B) Interaction of a tomato PP2CA-like phosphatase (5g052980) and Arabidopsis (left) or tomato (right) PYR/PYL ABA receptors. (C) Interaction of a tomato ΔN HAB1-like phosphatase (12g096020) and Arabidopsis (left) or tomato (right) PYR/PYL ABA receptors. (D) Elution profiles after size-exclusion chromatography of four tomato ABA receptors in the absence of ABA. The lines show the absorbance recorded at 280nm. Molecular mass markers are indicated in kDa. (This figure is available in colour at JXB online.)