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. 2014 May 26;65(15):4451–4464. doi: 10.1093/jxb/eru219

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — Overexpression of monomeric-type tomato receptors in Arabidopsis confers enhanced response to ABA and drought resistance. (A) Immunoblot analysis using antibody against the haemagglutinin (HA) tag shows expression of tomato ABA receptors in 21-day-old seedlings (two independent Arabidopsis T₃ transgenic lines for each tomato receptor). Ponceau staining is shown below. RBC indicates ribulose-1,5-bisphosphate carboxylase. (B) ABA-mediated inhibition of seedling establishment in transgenic lines compared with non-transformed Col plants and the hab1-1abi1-2 double mutant. * indicates P<0.05 (Student’s t-test) when comparing data of transgenic lines and the hab1-1abi1-2 mutant with non-transformed Col plants in the same assay conditions. Approximately 100 seeds of each genotype (three independent experiments) were sown on MS plates lacking or supplemented with 0.25 μM ABA. Seedlings were scored for the presence of both green cotyledons and the first pair of true leaves after 8 d. Values are averages ±SE. (C) Enhanced sensitivity to ABA-mediated inhibition of root growth of transgenic lines and the hab1-1abi1-2 mutant compared with non-transformed Col plants. Photographs show representative seedlings 10 d after the transfer of 4-day-old seedlings to MS plates lacking or supplemented with 5 μM ABA. Right panel: quantification of ABA-mediated root growth inhibition (values are means ±SE; growth of Col wild type on MS medium was taken as 100%). (D–F) Transgenic lines overexpressing monomeric-type receptors show enhanced drought resistance and survival, and higher RWC compared with non-transformed plants. (D) Two-week-old plants were deprived of water for 20 d and then re-watered. Photographs were taken at the start of the experiment (0-d), after 16 d and 20 d of drought, and 3 d after re-watering. (E) Percentage survival of non-transformed Col, hab1-1abi1-2, and transgenic lines 3 d after re-watering. (F) RWC of non-transformed Col, hab1-1abi1-2, and transgenic lines after 11, 14, and 17 d of water withdrawal. (This figure is available in colour at JXB online.)