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. 2014 Jul 14;11:128. doi: 10.1186/1743-422X-11-128

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Copyright © 2014 Tynell et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of leptomycin B treatment on NS1 intracellular localization. A549 cells were infected with wild-type A/Udorn/72 virus and incubated with or without 2 ng/ml LMB added at 3 h postinfection. 20 h postinfection the cells were fixed and the intracellular localization of NS1 protein and NP were visualized by immunofluorescence and estimated in a quantitative manner by observing 6–8 random frames under the microscope. The pictures were taken by Leica TCS NT confocal microscope. Data shown is representative of three independent experiments.