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. 2014 Jul 28;5:366. doi: 10.3389/fpls.2014.00366

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Copyright © 2014 Domínguez and Cejudo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TUNEL assay showing maternal tissues undergoing PCD at early and late stages of wheat seed development. In seeds at 5 DPA, nuclei from the nucellus and the integuments showed TUNEL-positive signal. Note the reduction of pericarp width and the formation of seed cuticles as a consequence of PCD (compare 5 DPA vs 25 DPA). Starch granules of different sizes are also observed in the starchy endosperm. P, pericarp; OI, outer integument; II, inner integument; NE, nucellar epidermis; NP, nucellar parenchyma; OP, outer pericarp; IP, inner pericarp; I, pigmented cuticle derived from outer and inner integuments; A, aleurone; E, starchy endosperm. Bars, 50 μm.