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. 2014 Mar 13;26(17):2704–2709. doi: 10.1002/adma.201304645

Figure 3.

image

 PC12 cells on Caf1 polymers. Glass slides were incubated with each of the proteins shown, used to culture rat pheochromocytoma PC12 cells for 24 h before being finally fixed and then imaged by scanning electron microscopy. Top; low magnification images to display differences in cell numbers on WT Caf1, Loop 5 RGDS Caf1 and collagen IV polymers. Lower images; comparison of cell morphology on the different polymers WT Caf1 polymer; Loop 5 RGDS Caf1 polymer; Collagen IV polymer; Loop 5 RGES polymer; Fibronectin (FN) and Buffer treated glass – no protein (NP) Histograms show differences in cell morphology. Non‐round cells show one or more filopodia. Data represent the mean of three experiments ± standard error of the mean (S.E.M). Significance was determined by one way ANOVA analysis with Scheffe as a post hoc test was conducted. All treatments were P < 0.001 compared to collagen IV.