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. 2014 Jul 21;20(27):9106–9115. doi: 10.3748/wjg.v20.i27.9106

Table 1.

Primer sets used for quantitative reverse transcriptase-polymerase chain reaction analysis of mouse colonic tissue

Gene name Accession No. Product size Direction Sequence (5’ to 3’) Ref.
Claudin2 NM_016675.4 120 bp Forward GGCGTCCAACTGGTGGGCTAC [42]
Reverse AACCGCCGTCACAATGCTGGC
Claudin3 NM_009902.4 132 bp Forward CAGGGGCAGTCTCTGTGCGAG [42]
Reverse GCCGCTGGACCTGGGAATCAAC
GAPDH NM_008084.2 132 bp Forward AACTTTGGCATTGTGGAAGG [42]
Reverse GGATGCAGGGATGATGTTCT
IL-1β NM_008361 73 bp Forward TCGCTCAGGGTCACAAGAAA [43]
Reverse CATCAGAGGCAAGGAGGAAAAC
INFγ NM_008337.3 93 bp Forward AGGTCCAGCGCCAAGCATTCAA [42]
Reverse AGCAGCGACTCCTTTTCCGCTT
iNOS U43428.1 76 bp Forward CAAAGTCTCAGACATGGCTTGC This study
Reverse TTCCTCTGTCAGGTCACTTTGG
NOX1 NM_172203.1 113 bp Forward CAGGCATCCTCATTTTGCGG This study
Reverse CCTTCTGCTGGGAGCGATAA
T-bet NM_019507.2 138 bp Forward CCACTGGATGCGCCAGGAAGTT [42]
Reverse TTCACCTCCACGATGTGCAGCC
TNF-α NM_013693.2 67 bp Forward TGGGACAGTGACCTGGACTGT [43]
Reverse TTCGGAAAGCCCATTTGAGT