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. 2014 Jul 28;5:357. doi: 10.3389/fpls.2014.00357

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Copyright © 2014 Hao, Avci, Tan, Zhu, Glushka, Pattathil, Eberhard, Sholes, Rothstein, Lukowitz, Orlando, Hahn and Mohnen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reduction in xylan deposition in anther endothecium cells in irx8 mutants. LR White-embedded WT (A) and irx8-5 (E) anther transverse sections (250 nm-thick) stained with toluidine blue O (Tol blue), arrowheads point to septum breakage. WT (B–D) and irx8 (F–H) anthers immunolabeled with xylan-reactive antibodies LM10 (B,F), LM11 (C,G), and CCRC-M149 (D,H). Bar = 50 μm for (A–H). TEM of WT (I) and irx8-5 (J) anther. Arrows point to the secondary wall thickenings in the endothecium cell layer, which is reduced in irx8-5. ep, epidermis; en, endothecium; p, pollen; lo, locule. Bar = 5 μm for (I) and (J).