Figure 11.1.

Flowchart for expression of vertebrate GPCRs in C. elegans. Generation of transient transgenic worms is carried out in three steps (Steps 1–3). After DNA constructs, expressing GPCR and DsRed are mixed (Step 1) and injected into a worm's gonad (Step 2), worms are cultured for two generations and then screened for those expressing the DsRed marker (Step 3). (White dots indicate worms expressing DsRed.) Selected transient transgenic worm lines are then exposed to UV light to generate integrated transgenic worm lines (Step 4). UV-exposed worms are cultured for four generations to establish stable integrated transgenic worm lines (Step 5).