Figure 11.4.

G_t activation by (b)Rho. Fluorescent assay of G_t activation by (b)Rho purified from bovine retina and (b)isoRho purified from transgenic worms expressing (b)opsin in muscles. Activation of G_t was monitored by the increase in intrinsic tryptophan fluorescence (λ_ex = 300 nm, λ_em = 345 nm) due to nucleotide exchange catalyzed by Rho. GTPγS (5 μM) was added to initiate the reaction after the signal was recorded for 300 s. Figure adapted with permission from Salom et al. (2012), The Federation of American Societies for Experimental Biology.