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. 2014 Jun 27;11:119. doi: 10.1186/1743-422X-11-119

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Copyright © 2014 Okeke et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Amplification and XbaI digestion of atip gene fragment. (A) PCR products of atip gene fragment were amplified with ATI-2 primer pairs. (B)XbaI digestion of atip gene fragment PCR products for 2 hours. (C). The XbaI digestion of atip gene fragment PCR products for 4 hours. Lanes (1) 1 kb plus DNA ladder, (2) CPXV-BR, (3) CPXV-No-H1, (4) CPXV-No-H2, (5) CPXV-No-F1, (6) CPXV-No-F2, (7) CPXV-Swe-H1, (8) CPXV-Swe-H2, (9) CPXV-FIN/T2000, (10) ECTV-MOS, (11) dH20.