Table 3.
Discordant results obtained during the clinical evaluation
| No. |
RT-PCR results |
Discordant analysis |
Interpretation | Comments | |||
|---|---|---|---|---|---|---|---|
| Real-time RT-PCR | EP-JV | EP-SR | Result | Genotype | |||
| 5 |
Neg. |
Neg. |
Pos. |
Neg. |
N/A |
FP (EP-SR) |
Non-specific amplification |
| 3 |
Neg. |
Pos. |
Neg. |
Neg. |
N/A |
FP (EP-JV) |
Non-specific amplification |
| 8 |
Pos. |
Neg. |
Neg. |
Pos. |
GII.7 |
FN (EP-JV and EP-SR) |
High viral loads (5.28 to 7.78 log10 copies/ml) |
| 3 |
Pos. |
Pos. |
Neg. |
Pos. |
GI.6 |
FN (EP-SR) |
High viral loads (5.29 to 5.74 log10 copies/ml) |
| 1 |
Pos. |
Pos. |
Neg. |
Pos. |
GI.3 |
FN (EP-SR) |
Low viral load (2.47 log10 copies/ml) |
| 1 | Pos. | Neg. | Pos. | Pos. | GII.15 | FN (EP-JV) | High viral load (6.33 log10 copies/ml) |
Abbreviations: FN false negative, FP false positive, N/A not applicable, Neg. Negative, No. number, Pos. positive.