Table 5.
Comparison of genotypes identified by SSCP and pyrosequencing
|
SSCP |
Pyprosequencing |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Genotype | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
|
1. WT/WT (n = 51) |
38 (74.5%) |
1 (1.9%) |
0.0 |
0.0 |
4 (7.8%) |
0.0 |
8 (15.6%) |
0.0 |
0.0 |
|
2. WT/MT (n = 92) |
0.0 |
88 (95.6%) |
2 (2.2%) |
0.0 |
0.0 |
0.0 |
0.0 |
2 (2.2%) |
0.0 |
|
3. MT/MT (n = 39) |
0.0 |
6 (15.3%) |
27 (69.2%) |
6 (15.4%) |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
|
4. (n = 1) |
0.0 |
0.0 |
0.0 |
1.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
| n = 183 | n = 183 | ||||||||
For the SSCP EAAT2F and EAAT2R primers were used and pyrosequencing was done with EAAT2PyroSeq1 primer (Figure 1 and Table 1). The genotypes that were correctly identified by both methods are indicated in bold. The genotype numbers indicated under pyrosequencing corresponds to those used in Table 2.