Table 6.
Comparison of genotypes identified by SSCP and pyrosequencing
|
SSCP |
PYROSEQUENCING |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Genotype | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
|
1. WT/WT (n = 3) |
2 (66.7%) |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
1 (33.3%) |
0.0 |
0.0 |
|
2. WT/MT (n = 11) |
0.0 |
9 (81.8%) |
1 (9.1%) |
0.0 |
0.0 |
0.0 |
1 (9.1%) |
0.0 |
0.0 |
|
3. MT/MT (n = 1) |
0.0 |
0.0 |
1 (100%) |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
| n = 15 | n = 15 | ||||||||
For SSCP EAAT2F and EAAT2Pyro primers were used and pyrosequencing was done with EAAT2PyroSeq1 primer. The genotypes that were correctly identified by both methods are indicated in bold.