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. Author manuscript; available in PMC: 2014 Jul 28.
Published in final edited form as: Nat Neurosci. 2014 Mar 2;17(4):513–521. doi: 10.1038/nn.3668

Figure 3.

Figure 3

PU.1 and PU.1-C/EBPs target genes are upregulated in primary microglia from R6/2 mice. (a) PU.1 expression in P0 primary microglia purified from nontransgenic littermates and R6/2 pups. Immunoblot represents lysates of pool of microglia cells obtained from 5 pups per group. Image shown has been cropped from the original one. Full-length blot is presented in Supplementary Figure 8. (b) IL6 protein secretion in culture supernatant from P0 primary microglia from R6/2 pups and nontransgenic littermates. Graph represents pg/ml of IL6 in pooled microglia culture supernatants from 5 pups respectively in each biological replicate, (n= 3 biological replicates, n.d.=not detected). qRT-PCR analysis for Il6 (c) and Tnαf (d) mRNAs expression levels in presence of C/ebpα,β or Spfi1 siRNAs respectively in nontransgenic littermates and R6/2 primary microglia (mean±sd, n= 3 biological replicates). In all cases p values were calculated using the two-tailed paired student t-test. Each experiment is representative of at least three independent replicates.