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. Author manuscript; available in PMC: 2014 Jul 28.
Published in final edited form as: Nat Neurosci. 2014 Mar 2;17(4):513–521. doi: 10.1038/nn.3668

Figure 4.

Figure 4

PU.1 and PU.1-C/EBPs target genes are up-regulated in primary microglia from Hdh175/175 Knock-in mice. (a) qRT-PCR analysis for Spfi1 (PU.1) mRNA and protein expression in primary microglia purified from Hdh7/7, Hdh175/7 and Hdh175/175 newborn (P0) pups (mean±sd, n= 3 biological replicates). Image shown has been cropped from the original one. Full-length blot is presented in Supplementary Figure 8. (b) qRT-PCR analysis for Il6, Tnfα, Irf1 and Tlr2 mRNAs expression in primary microglia purified from Hdh7/7, Hdh175/7 and Hdh175/175 pups (mean±sd, n= 3 biological replicates). All p values were determined by two-tailed paired student t-test. (c) Gene Ontology analysis reporting the biological processes enriched in genes up-regulated in Hdh175/175 versus Hdh7/7 for microglia and BMDM obtained from adult mice (18–20 months old). RNAseq results are based on mRNA extraction from pooled microglia cells or BMDM obtained from 4 mice per group.