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. 2014 Jun 2;13:210. doi: 10.1186/1475-2875-13-210

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Copyright © 2014 Le et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Inhibition of plugin cross-linking by DHI inhibitors. Fluorescent image of SDS-PAGE for FITC-cadaverine labeling and cross-linking of plugin by AgTG3. Lanes (1–4): (1) AgTG3, (2) Plugin-C, (3) AgTG3 and plugin-C, (4) AgTG3, plugin-C, and 5 mM CaCl₂. Lanes (5–8): AgTG3, plugin-C, 5 mM CaCl₂ and (5) 10 mM EDTA, (6) 50 mM IA, (7) 100 μM 1, (8) 100 μM 3. (below) Coomassie stain indicating relative levels of monomeric Plugin-C.