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. Author manuscript; available in PMC: 2015 Jun 19.
Published in final edited form as: Cell. 2014 Jun 19;157(7):1605–1618. doi: 10.1016/j.cell.2014.04.033

Figure 7. Genome-wide Analysis to Identify Targets of Translational Readthrough.

Figure 7

(A) Properties of candidate genes selected for experimental validation. Candidates were selected using a bioinformatic screening protocol (Figure S6).

(B) Validation of translational readthrough targets. Chimeric plasmids containing about 700 nt of the 3′-terminus of human coding sequences upstream of the inter-stop codon region and in-frame with FLuc were transfected into HEK293 cells. Relative FLuc activities normalized to FLuc mRNA were determined by qRT-PCR (top). Readthrough efficiency is expressed as percent of FLuc activity of normal translation controls in which canonical UGA stop codon is replaced by GCA (bottom).

See also Figure S6.