Animals injected in the PVH with a DSAP show a significant loss of DBH (A and Cb) and PNMT (B and Db) immunoreactive neurons in the AP and NTS compared with animals injected with a MIgG-SAP (Ca and Da). Animals were exposed to either rapid-onset (open bars) or slow-onset (solid bars) hyperinsulinemic-hypoglycemic clamp. Results in A and B are expressed as mean (SEM) number of labeled cells per section. ns, not significant; c, central canal, spinal cord/medulla; co, commisural part of the NTS; ge, gelatinous part of the NTS; m, medial part of the NTS; ts, solitary tract. Scale bar = 200 μm.