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Strategy for using heavy methyl-SILAC labeling to study dynamics of histone methylation. HeLa cells are introduced into the heavy methionine-labeled medium to allow for incorporation of the heavy methyl group on histones. Cells are harvested at a series of time points and subsequently histones are extracted. Bulk histone proteins or individual histone variant separated by HPLC is sequentially subjected to propionylation, trypsin digestion and LC-MS/MS analysis.
