Figure 4.

Centrosome amplification induces the formation of invasive protrusions. (a) Still images from videos of MCF10A cells plated in three-dimensional cultures for 4 days. MCF10A cells form spheroids, acini, when grown in three dimensions, whereas MCF10A cells with extra centrosomes form invasive protrusions in three-dimensional cultures (black arrow). Nuclei, labelled with H2B-GFP (green) can migrate into an invasive protrusion (white arrow). Time-scale, hour : min. (b) Scheme of normal and invasive acini. Note that invasive protrusions form at sites where the basement membrane is (BM) degraded.