Figure 5.

Ad-IRF-1 is necessary and responsible for inducing AS in CEACAM1 to generate the L-isoform. (A) Schematic diagram of CEACAM1’s promoter (WT; upper black arrow) and mutant GG- > CC translationally fused to exons 6-7-8 minigene. Only AS to generate the L-isoform expresses the GFP reporter. Mutations that ablate the hnRNP L and hnRNP A1 binding sites on exon 7 (black box) are indicated (ΔLΔA1). A mCherry reporter that was co-transfected and used as a quantitation control was fused to CEACAM1 WT ISRE (lower black arrow). (B-C) Flow cytometric analyses, histogram and statistical analyses of Q2 in Figure 5B were similar to description in Figure 2 legend.