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. 2014 Apr 28;13:91. doi: 10.1186/1476-4598-13-91

Figure 2.

Figure 2

ETPs disrupt the endogenous HIF-1α/p300 complex in cells. PC3 cells were cultured under normoxia or hypoxia (CoCl2) in the presence or absence of the indicated concentrations of gliotoxin and chaetocin. After 18 h, co-immunoprecipitations were performed from the cell lysates using a p300 mouse monoclonal antibody and Protein A/G Agarose. Bound proteins were eluted with SDS buffer followed by immunoblot analysis of the lysates (left panels) and immune complexes (right panels) with antibodies recognizing p300 and HIF-1α. A, Treatment with 25 nM and 500 nM gliotoxin (GLIO). B, Treatment with 5 nM and 100 nM chaetocin (CTM) (n = 3 for all).