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. 2014 Apr 28;13:91. doi: 10.1186/1476-4598-13-91

Figure 4.

Figure 4

ETPs decrease expression of HIF-1α-dependent target genes. PC3 cells were seeded into 6-well plates and incubated for 18 h under normoxic or hypoxic conditions, in the absence or presence of the indicated concentrations of gliotoxin (top panel), chaetocin (middle panel), or chetomin (bottom panel). Total RNA was harvested and tested for VEGF, LDHA, and ENO1 mRNA expression by qPCR, as described in the Materials and methods. Results are expressed as fold increase relative to mRNA levels under hypoxic conditions in the absence of ETPs. β-actin was tested in parallel as an internal control for input RNA. Results are the mean ± S.E.M of independent experiments run in triplicate (n = 2-5). A repeated measures ANOVA was performed on the data with Hochberg’s post-hoc method. *, p < 0.05, **, p < 0.001, ***, p < 0.0001.