Figure 4.

Down-regulation of RasGRP3 increases the sensitivity to tamoxifen and trastuzumab of the T-47D cell line. The indicated MCF7 and T-47D derived cell lines were seeded at a density of 1 x 10⁴ cells/ well and treated with tamoxifen (A) and trastuzumab (B) (tamoxifen: MCF7 cells: 0–200 μM; T-47D cells: 0–100 μM; trastuzumab: both MCF7 and T-47D cells: 0–10 μM). After 72 hours of incubation cell proliferation was determined using the CyQuant GR cell proliferation assay. The results were normalized to the levels of untreated cells. Values represent the mean ± SEM of 2 independent experiments.