Table 1.

Temporal classification of up-regulated retinal gene cluster changes following ONC

Gene ontology	Clusters	Time point	P value
Molecular function	Structural eye protein	3 dpc	1.90E-06
	Eye development	3 dpc	4.50E-03
	Extracellular matrix binding	3 dpc	5.30E-03
	Calcium ion binding	7 dpc	3.30E-02
	Structural eye lens protein	21 dpc	2.20E-11
	Structural molecular activity	21 dpc	4.60E-06
Biological process	Response to wounding	3 dpc	1.00E-04
	Inflammatory response	3 dpc	3.80E-04
	Defense response	3 dpc	5.90E-04
	Positive regulation of immune system response	3 dpc	1.20E-02
	Rho protein signal transduction	3 dpc	5.90E-03
	Regulation of signal proliferation	3 dpc	2.70E-02
	Defense response	7 dpc	5.40E-04
	Inflammatory response	7 dpc	1.60E-02
	Response to wounding	7 dpc	4.70E-02
	Sensory perception	14 dpc	8.80E-03
	Neurological system process	14 dpc	2.30E-02
	G-protein coupled receptor signaling pathway	14 dpc	3.90E-02
	Macromolecular complex assembly	28 dpc	1.30E-02
	DNA packaging	28 dpc	3.10E-02
	Positive regulation of protein kinase activity	28 dpc	4.50E-02
Cellular component	Extracellular region part	3 dpc	3.20E-03
	Extracellular matrix	3 dpc	7.60E-03
	Lysosome	3 dpc	3.40E-02
	Extracellular region part	7 dpc	3.50E-02
	Microsome	14 dpc	1.70E-02
	Intermediate filament	14 dpc	3.70E-02
	Ribosome	21 dpc	5.00E-03

Gene expression fold-change values were grouped individually from naïve eyes and ONC eyes out to 28 days post crush (dpc). Genes were highlighted based on fold values for up-regulated (≥1.5) retinal datasets. The selected genes were analyzed by gene ontology (GO) based cluster identification at each time point using DAVID. Significance was determined using the Benjamini multiple test correction, GO enrichment score χ² test and Fishers Exact test (p < 0.05).