Table 2.
Temporal classification of down-regulated retinal gene cluster changes following ONC
| Gene ontology | Clusters | Time point | P value |
|---|---|---|---|
| Molecular function |
Structural eye lens protein |
7 dpc |
3.80E-15 |
| |
Structural eye lens protein |
28 dpc |
2.60E-14 |
| |
Pattern binding |
28 dpc |
3.80E-02 |
| Biological process |
Chromatin assembly |
7 dpc |
4.80E-04 |
| |
Regulation of axonogenesis |
21 dpc |
7.40E-03 |
| |
G-protein coupled receptor signaling pathway |
21 dpc |
2.00E-04 |
| |
Neurological system process |
21 dpc |
7.10E-03 |
| |
Intermediate filament bundle process |
21 dpc |
6.40E-05 |
| |
Microtubule based process |
21 dpc |
5.10E-03 |
| |
Axonogenesis |
21 dpc |
1.70E-02 |
| |
Neuron projection morphogenesis |
21 dpc |
2.00E-02 |
| |
Neuron differentiation |
21 dpc |
4.20E-02 |
| |
Cell morphogenesis involved in differentiation |
28 dpc |
1.20E-02 |
| Cellular component |
Nucleosome |
7 dpc |
7.50E-05 |
| |
Neuron projection |
21 dpc |
5.00E-05 |
| |
Axon |
21 dpc |
6.50E-05 |
| |
Neurofilament |
21 dpc |
1.60E-04 |
| |
Intrinsic to membrane |
21 dpc |
1.30E-02 |
| |
Neuron projection |
28 dpc |
9.90E-03 |
| Chromosome | 28 dpc | 3.10E-02 |
Gene expression fold-change values were grouped individually from naïve eyes and ONC eyes out to 28 days post crush (dpc). Genes were highlighted based on fold values for down-regulated (≤ −1.5) retinal datasets. The selected genes were analyzed by gene ontology (GO) based cluster identification at each time point using DAVID. Significance was determined using the Benjamini multiple test correction, GO enrichment score χ2 test and Fishers Exact test (p < 0.05).