Table 2.
Unique sequences of miRNA isoforms (isomiRs) and other sncRNAs (with RPM ≥100) that underwent significant (qFDR < 0.05) differential up- or down-regulation following SNL nerve injury in HA vs. LA rats
| Small RNA type and sequence | Mean RPM | Mean RPM | SNL-reg | diff-reg | |||
|---|---|---|---|---|---|---|---|
| (canonical miRNA or RNA type) |
(sham pools) |
(SNL pools) |
subtraction method (SNL/sham fold-change) |
|
|||
| |
HA |
LA |
HA |
LA |
HA |
LA |
HA-LA |
|
unique-sequence miRNA isoform (isomiR), all pools, and those added by HA_SNL1 pool exclusion * | |||||||
| UAUAGUACUGUGAUAACUGACU (rno-miR-101a-3p) |
11.7 |
9.3 |
9.9 |
11.3 |
−0.167 (−1.182) |
0.194 (1.215) |
−0.361 (0.397) |
| CAGUGCAAUGUUAAAAGGGC (rno-miR-130a-3p) * |
38.0 |
41.9 |
48.2 |
45.0 |
0.237 (1.268) |
0.071 (1.074) |
0.166 (0.194) |
| UCCCUGAGACCCUAACUUG (rno-miR-125b-5p) * |
47.8 |
30.4 |
35.5 |
33.1 |
−0.295 (−1.346) |
0.085 (1.089) |
−0.380 (0.435) |
| UGGACGGUGUGAGGCC (sha-miR- 5105) * |
11.0 |
15.6 |
13.5 |
11.9 |
0.204 (1.227) |
−0.269 (−1.311) |
0.473 (0.537) |
|
unique-sequence sncRNAs with RPM ≥100, after HA_SNL1 pool exclusion | |||||||
| AGCCGCCUGGAUACCGCAGCUAGGAAUAA (rRNA) |
1843.3 |
616.5 |
548.0 |
666.0 |
−1.846 (−3.363) |
0.078 (1.080) |
−1.924 (2.443) |
| GCAUUAGUGGUUCAGUGG (tRNA) |
621.1 |
719.9 |
771.1 |
713.5 |
0.215 (1.242) |
−0.009 (−1.009) |
0.224 (0.251) |
| UCAUUGGUGGUUCAGUGG (tRNA) | 88.6 | 111.5 | 114.1 | 103.4 | 0.252 (1.288) | −0.075 (−1.078) | 0.327 (0.366) |