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. 2014 Jul 28;9(7):e102535. doi: 10.1371/journal.pone.0102535

Figure 4. Inhibition of autophagy or over-expression of β-catenin attenuates the effects of resveratrol on BCSCs.

Figure 4

(A) Western blot analysis of β-catenin and cyclin D1 in BCSCs treated with different dose of resveratrol for 24 h. (B) Immunohistochemistry detection of β-catenin and cyclin D1 in xenografted breast tumors. (C) The effects of resveratrol treatment for 24 h on ALDH-positive populations in SUM159 cells and SUM159 cells co-treated with 10 µM CQ (CQ-SUM). (D) The effects of resveratrol treatment for 24 h on autophagy in BCSCs, BCSCs co-treated with 10 µM CQ (CQ-CSCs) and BCSCs transfected with pcDNA3-S33Y β-catenin (TCβ-catenin). (E) The effects of resveratrol treatment for 24 h on cells viability in BCSCs, CQ-CSCs and TCβ-catenin cells. (F) Western blotting analysis of β-catenin and cyclin D1 in TCβ-catenin cells treated with different dose of resveratrol for 24 h. Data are presented as mean ± SD (n = 3). * P<0.05, ** P<0.01 compared with the control (0 µM); # P<0.05, ## P<0.01 compared with corresponding group in CSCs.