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. 2014 Jul 28;9(7):e102232. doi: 10.1371/journal.pone.0102232

Table 3. Additional lactamases detected by the microarray in comparison to conducted control PCRs.

Primer forward Sequence Primer reverse Sequence lengths (bp) Reference No. of Isolates No. of Isolates % Concordance
Ma PCRb
blaOXA-1 prim_oxa1_20_fwd 5′-TTCTGTTGTTTGGGTTTCGC-3′ prim_oxa1_20 5′-ACGCAGGAATTGAATTTGTTC-3′ 190 this study 16 16 100
blaOXA-2 prim_oxa2_10_fwd 5′-GTTAATGGCAATCCGAATCTT-3′ prim_oxa2_10 5′-GGATCGTGCCATGTTGG-3′ 176 this study 4 4 100
blaOXA-9 prim_oxa9_10_fwd 5′-TGTGTCTCCGTGCTCGTCTT-3′ prim_oxa9_10 5′-CCGATCAACTCCCAGACG-3′ 207 this study 33 33 100
blaOXA-10 prim_oxa7_10_fwd 5′-TGGAACAAAGAGTTCTCTGCC-3′ prim_oxa7_10 5′-GGCTTTCCGTCCCATTTG-3′ 207 this study 3 3 100
blaOXA-51 lb_oxa_654_fwd 5′-AGCCTGCTCACCTTATATAGTGACT-3′ lb_oxa_654 5′-TTGAAGGTCGAAGCAGGTA-3′ 201 this study 25 25 100
blaOXA-58 lb_oxa_657_fwd 5′-GCCAATGCACTAATTGGTTTAGA-3′ lb_oxa_657 5′-GCAATTCACTTTGCATTAAGCT-3′ 193 this study 2 2c 100
blaCTX-M1/15 prim_ctxM1_10_fwd 5′-AGGCGTTTTGACAGACTATTCA-3′ prim_ctxM1_10 5′-CCGTTTGCGCATACAGC-3′ 152 this study 17 17 100
blaVIM DG_VIM-cons_fwd 5′-GATGGYGTTTGGTCGCATATCKCAAC-3′ DG_VIM-cons_rev 5′-CGAATGCGCAGCACCRGGATAGAA-3′ 390 this study 1 1d 100
Total concordance 101 101 100
a

Results were obtained using the microarray-based carbapenemases array, designed in this study.

b

Results were obtained by PCR designed in this study.

c

One blaOXA-58 was detected in an OXA-23 negative strain tested by microarray, which was originally tested as an OXA-23 positive strain by the national reference center.

d

BlaVIM was detected in addition to an IMP carbapenemase gene.