Table 3. Additional lactamases detected by the microarray in comparison to conducted control PCRs.
Primer forward | Sequence | Primer reverse | Sequence | lengths (bp) | Reference | No. of Isolates | No. of Isolates | % Concordance | |
Ma | PCRb | ||||||||
blaOXA-1 | prim_oxa1_20_fwd | 5′-TTCTGTTGTTTGGGTTTCGC-3′ | prim_oxa1_20 | 5′-ACGCAGGAATTGAATTTGTTC-3′ | 190 | this study | 16 | 16 | 100 |
blaOXA-2 | prim_oxa2_10_fwd | 5′-GTTAATGGCAATCCGAATCTT-3′ | prim_oxa2_10 | 5′-GGATCGTGCCATGTTGG-3′ | 176 | this study | 4 | 4 | 100 |
blaOXA-9 | prim_oxa9_10_fwd | 5′-TGTGTCTCCGTGCTCGTCTT-3′ | prim_oxa9_10 | 5′-CCGATCAACTCCCAGACG-3′ | 207 | this study | 33 | 33 | 100 |
blaOXA-10 | prim_oxa7_10_fwd | 5′-TGGAACAAAGAGTTCTCTGCC-3′ | prim_oxa7_10 | 5′-GGCTTTCCGTCCCATTTG-3′ | 207 | this study | 3 | 3 | 100 |
blaOXA-51 | lb_oxa_654_fwd | 5′-AGCCTGCTCACCTTATATAGTGACT-3′ | lb_oxa_654 | 5′-TTGAAGGTCGAAGCAGGTA-3′ | 201 | this study | 25 | 25 | 100 |
blaOXA-58 | lb_oxa_657_fwd | 5′-GCCAATGCACTAATTGGTTTAGA-3′ | lb_oxa_657 | 5′-GCAATTCACTTTGCATTAAGCT-3′ | 193 | this study | 2 | 2c | 100 |
blaCTX-M1/15 | prim_ctxM1_10_fwd | 5′-AGGCGTTTTGACAGACTATTCA-3′ | prim_ctxM1_10 | 5′-CCGTTTGCGCATACAGC-3′ | 152 | this study | 17 | 17 | 100 |
blaVIM | DG_VIM-cons_fwd | 5′-GATGGYGTTTGGTCGCATATCKCAAC-3′ | DG_VIM-cons_rev | 5′-CGAATGCGCAGCACCRGGATAGAA-3′ | 390 | this study | 1 | 1d | 100 |
Total concordance | 101 | 101 | 100 |
Results were obtained using the microarray-based carbapenemases array, designed in this study.
Results were obtained by PCR designed in this study.
One blaOXA-58 was detected in an OXA-23 negative strain tested by microarray, which was originally tested as an OXA-23 positive strain by the national reference center.
BlaVIM was detected in addition to an IMP carbapenemase gene.