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. 2014 Jun 11;4(13):2714–2737. doi: 10.1002/ece3.1126

Figure A7.

Figure A7

RFLP pattern of PCR products of rrs gene of the Wolbachia supergroup O and B in Bemisia tabaci corresponding to VspI (AseI) digestion. The different profiles were obtained from individuals representing different Wolbachia in B. tabaci. The bands shown on the bottom are primer dimers. Lane 1, undigested Wolbachia O obtained by PCR from Asia II 1; lane 2–4, undigested Wolbachia B obtained by PCR from Asia II 7, Asia I and China 1, respectively; lane 5–6, Wolbachia PCR amplified production from MEAM1 and MED as controls; lane 8, digested Wolbachia O obtained by PCR from Asia II 1; lane 9–11, Wolbachia B obtained by PCR from Asia II 7, Asia I and China 1, respectively; lane 12–13, Wolbachia PCR amplified production from MEAM1 and MED as controls; lane M, DNA size markers (100, 250, 500, 750, 1000, and 2000 bp from bottom to top).