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In situ hybridization of zAe3 mRNA in early development. In situ hybridization reveals specific Ae3 mRNA expression in fin buds of 36 and 72 hpf embryos (arrowheads, upper panels) that was not detected using Ae3 sense strand control probe (lower panels). Fin bud expression of Ae3 mRNA persisted at 96 hpf (not shown). Specific in situ hybridization signals were not detected in brain, heart or retina
